João A. Lopes

Associate Researcher
REQUIMTE/University of Porto
ICETA-Instituto de Ciências e Tecnologias Agrárias e Agro-Alimentares da UP
Phone: +351 220428664


Assistant Professor
Department of Pharmaceutics and Pharmaceutical Technology
Faculty of Pharmacy, Univ. of Lisbon
Av. Prof. Gama Pinto, 1649-003 Lisbon, Portugal
Tel. +351 217946400 Ext. 14213, Fax. +351 217946470
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Short CV

João Almeida Lopes (JAL) graduated in Chemical Engineering (biotechnology branch) in 1997 at Instituto Superior Técnico (Technical University of Lisbon). In 2001, in the same institution, he completed the PhD in Chemical Engineering in the area of chemometrics applied to the pharmaceutical industry. This PhD Thesis has been awarded with the CUF prize (for the best chemical engineering Thesis of the period 1999-2001). In 2004, during a PostDoc research period, JAL was awarded with the first prize in the entrepreneurship contest Bioempreendedor where infrared spectroscopy coupled with chemometrics was proposed for rapid and cost effective identification of human bacteria pathogens. JAL joined the Associated Laboratory REQUIMTE and University of Porto in 2006 as Assistant Researcher in chemometrics and process spectroscopy. In 2006, he started WORLDMETRICS, a company for industrial implementation of advanced process analytical technology (PAT) solutions. Since 2014, JAL is Assistant Professor of Pharmaceutical Technology at the Faculty of Pharmacy of University of Lisbon. JAL research activities are based on chemometrics and vibrational spectroscopy in the pharmaceutical and agrofood fields. He started the Chemometrics & Process Analytical Technology unit of the GABAI/University of Porto research centre. This research unit is focused on Process Analytical Technology (PAT) and Quality-by-Design approaches for exact, life and environmental sciences. He’s member of the steering committee of the European Federation of Pharmaceutical Sciences Quality-by-Design/PAT network since 2010. He coordinated and participated in more than 35 funded projects of different topologies most in collaboration with industrial partners mainly primary and secondary pharmaceutical, oil refining, agrofood, dairy and brewing industries). He currently holds 5 R&D contracts with pharmaceutical and agrofood companies. He supervised several undergraduate students, PhD students and Postdoc researchers. He published 64 peer-reviewed articles in international scientific journals and book chapters. He lectured several invited communications in international conferences and symposia, co-authored 65 oral communications and 96 poster communications in international scientific conferences. JAL is a member founder ofthe National Science and Technology Researchers Association and President in 2013/2014.

Research Topics

Current research activity is focused on the development and application of chemometrics and vibrational spectroscopy  (near-infrared, mid-infrared) in the context of green-chemistry (non-destructive, non-invasive, reagent-free): 1) Chemometrics modelling; 2) Bioreactors monitoring and control, 3) pharmaceutical processes and pharmaceutical quality control, 4) analytical methods development and optimization, 5) Food quality control; 6) bacteria high-throughput identification; 7) Conservation&restauration studies

Publications (last 3 years)

Book chapters
Andrea Catalina Galvis-Sánchez, João Almeida Lopes, Ivonne Delgadillo and António O.S.S. Rangel. Chapter 26: Sea salt, pag. 719. In: Comprehensive analytical chemistry, Vol. 60. Food Protected designation of origin: methodologies and applications. Edited by Miguel de la Guardia M and Ana Gonzalvez, Elsevier, UK. 2013. (ISBN: 978-0-444-59562-1).

Clara C. Sousa and João A. Lopes. Chapter 20: Infrared spectroscopy detection coupled to chemometrics to characterize foodborne pathogens at a subspecies level, pag. 385. In: Mathematical and Statistical Approaches in Food Science and Technology. Edited by Daniel Granato and Gáston Ares,IFT Press – Wiley-Blackwell.UK. 2014. (ISBN: 978-1-118-43368-3)

Sarraguça M.C., Santos J.R., Magalhães L.M., Rangel A.O.S.S., Lopes J.A. Chapter X: Use of near infrared spectroscopy for coffee beans quality assessment, pag. XX. In: Coffee in Health and Disease Prevention. Edited by Victor R. Preedy, Elsevier,Amsterdam. 2013 (in-print)

Peer-reviewed scientific articles 

Sílvia Mas, Catarina Miguel, Maria João Melo, João A. Lopes, Anna de Juan, Screening and quantification of proteinaceous binders in medieval paints based on micro-fourier transform infrared spectroscopy and multivariate curve resolution alternating least squares, Chemom. Intell. Lab Syst., in-print

Clara Sousa, Liliana Silva, Filipa Grosso, João P. Lopes, Luísa Peixe (2014) Development of a FTIR-ATR based model for typing clinically relevant Acinetobacter baumannii clones belonging to ST98, ST103, ST208 and ST218, Journal of Photochemistry and Photobiology B: Biology, 133, 108-114 [doi: 10.1016/j.jphotobiol.2014.02.015]

R. Matias, P.R.S. Ribeiro, M.C. Sarraguça, J.A. Lopes (2014) A UV spectrophotometric method for the determination of folic acid in pharmaceutical tablets and dissolution tests, Anal. Met., 6(9), 3065-3071 [doi:10.1039/C3AY41874J].

Sousa C, Silva L, Grosso F, Nemec A, Lopes J, Peixe L. Discrimination of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex species by Fourier Transform Infrared Spectroscopy, European Journal of Clinical Microbiology and Infectious Diseases, in-print

Susana A Lopes,Jorge MG Sarraguça: João A Lopes Maria E Duarte, A new approach to talent management in law firms: integrating performance appraisal and assessment center data, International Journal of Productivity and Performance Management, in.print

Ângela Novais, Clara Sousa, Juan de Caballero, Ana Fernandez-Olmos, João Lopes, Maria Helena Ramos, Teresa M Coque, Rafael Cantón, Luísa Peixe, MALDI-TOF mass spectrometry as a tool for discrimination of high-risk Escherichia coli clones from phylogenetic groups B2 (ST131) and D (ST69, ST405, ST393), European Journal of Clinical Microbiology & Infectious Diseases, in-print

Saraiva R, Lopes JA, Feio MJ, Gameiro P*, Discrimination of single-porin Escherichia (E.) coli mutants by ATR and transmission mode FTIR spectroscopy, J. Biophotonics, in-print [doi: 10.1002/jbio.201200131]

Sousa CCS, Grosso F, Meirinhos-Soares L, Peixe L, Lopes JA*, Identification of carbapenem-resistant A. baumannii clones using infrared spectroscopy, J. Biophotonics, in-print [doi: 10.1002/jbio.201200075]

Couto M.C.M., Pinto I., Madureira T.V., Rocha M.J., Tiritan M.E., Lopes J.A. and Almeida A.A. (2014), Lower Douro river basin (Portugal) water quality – Focus on trace element changes and anthropogenic sources of contamination, Global NEST Journal,16(2), 251-267

Clara Sousa, Ângela Novais, Ana Magalhães, João Lopes, and Luisa Peixe, Diverse high-risk B2 and D Escherichia coli clones depicted by Fourier Transform Infrared Spectroscopy (2013), Sci. Rep., 3, 3278 [doi:10.1038/srep03278]

A.F.T. Silva, A. Burggraeve, Q. Denon, P. Van der Meeren, N. Sandler, T. Van Den Kerkhof, M. Hellings, C. Vervaet, J.P. Remon, J.A. Lopes, T. De Beer (2013) Particle sizing measurements in pharmaceutical applications: comparison of in-process methods versus offline methods, Eur. J. Pharm. Biopharm., 85, 1006-1018 [doi: 10.1016/j.ejpb.2013.03.032]

C. A. Teixeira dos Santos, M. Lopo, R.N.M.J. Páscoa, J.A. Lopes* (2013) A review on the applications of portable near infrared spectrometers in the agro-food industry, Appl. Spectrosc., 67(11), 1215-1233. [doi:10.1366/13-07228]

Ana Serrano, Micaela Sousa, Jessica Hallett, Monique S. J. Simmonds, Mark Nesbitt, and João A. Lopes* (2013) Identification of Dactylopius cochineal species with high-performance liquid chromatography and multivariate data analysis, Analyst, 2013, 138 (20), 6081 – 6090 [doi:10.1039/C3AN00052D]

Sarraguça MC, Santos JR, Rangel AOSS, Lopes JA* (2013) Authenticity control of roasted coffee brands with near infrared spectroscopy, Food Anal. Met., 6(3),892-899 [doi:10.1007/s12161-012-9499-y]

Vaz MM, Meirinhos-Soares, Sousa CCS, Ramirez M, Melo-Cristino J, Lopes JA* (2013) Serotypes discrimination of encapsulated Streptococcus pneumoniae strains by Fourier-transform infrared spectroscopy and chemometrics, J. Microbiol. Met., 93, 102-107

Galvis-Sánchez AC, Santos IC, Mesquita RBR, Lopes JA, Rangel AOSS, Delgadillo I* (2013) Application of Mid- and Near-Infrared Spectroscopy for the Control and Chemical Evaluation of Brine Solutions and Traditional Sea Salts, Food Anal. Met., 6, 470-480 [doi: 10.1007/s12161-012-9458-7]

Páscoa RNMJ, Magalhães LM, Lopes JA* (2013) FT-NIR spectroscopy as a tool for valorization of spent coffee grounds: application to assessment of antioxidant properties, Food Res. Int., 55, 579-586 [doi:10.1016/j.foodres.2013.01.035]

Miguel CP, Lopes JA*, Clarke M, Melo MJ (2012) Combining infrared spectroscopy with chemomeric analysis for the characterization of proteinaceous binders in medieval paints, Chemom. Intell. Lab. Syst., 119, 32-38 [doi: 10.1016/j.chemolab.2012.09.003]

Aksu B, De Beer T, Folestad S, Ketolainen J, Lindén H, Lopes JA, de Matas M, Oostra W, Rantanen J, Weimer M (2012) Strategic funding priorities in the pharmaceutical sciences allied to Quality by Design (QbD) and Process Analytical Technology (PAT), Eur. J. Pharma. Sci., 47, 402-405 [doi: 10.1016/j.ejps.2012.06.009]

Santos JR, Sarraguça MC, Rangel AOSS, Lopes JA* (2012) Evaluation of green coffee quality using NIR spectroscopy: a quantitative approach, Food Chem., 135, 1828-1835 [doi: 10.1016/j.foodchem.2012.06.059]

Lourenço ND, Lopes JA, Almeida CF, Sarraguça MC, Pinheiro HM* (2012) Bioreactor monitoring with spectroscopy and chemometrics: a review, Anal. Bioanal. Chem., 404, 1211-1237 [doi:10.1007/s00216-012-6073-9]

Ribeiro DSM, Lopes JA, Santos JLM, Prior JAV* (2012) Exploiting adsorption and desorption at solid-liquid interface for the fluorometric monitoring of glibenclamide in adulterated drinks, Anal. Chim. Acta, 721, 97-103 [doi: 10.1016/j.aca.2012.01.043]

Lopes SB, Sarraguça JMG, Prior JAV, Lopes JA* (2012) Development of an HPLC assay methodology for a desonide cream with chemometrics assisted optimization, Anal. Lett., 45(11), 1390-1400  [10.1080/00032719.2012.675494]

Sarraguça JMG*, Lopes JA, Santos JLM, Lima JLFC (2012) Mathematical simulation of signal profiles in flow analysis, Anal.Lett., 45(1), 85-98 [10.1080/00032719.2011.582551]

Preisner OE, Menezes JC, Guiomar R, Machado J, Lopes JA* (2012) Discrimination of Salmonella enterica serotypes by Fourier transform infrared spectroscopy, Food Res. Int., 45(2), 1058-1064 [doi:10.1016/j.foodres.2011.02.029]

Galvis-Sánchez AC* , Lopes JA, Delgadillo I, Rangel AOSS (2011)  Fourier-transform near-infrared spectroscopy application for sea salt quality evaluation, J. Agric. Food Chem., 59(10), 11109-11116 [doi:10.1021/jf202204d]

Serrano A, Sousa MM*,  Hallett J, Lopes JA, Oliveira C (2011) Analysis of natural red dyes (cochineal) in textiles of historical importance using HPLC and multivariate data analysis, Anal. Bioanal. Chem., 401, 735-743 [doi:10.1007/s00216-011-5094-0]

Sarraguça MC, Lopes JA* (2011) A near-infrared spectroscopy method to determine aminoglycosides in pharmaceutical formulations, Vib. Spectrosc., 56, 184-192 [doi:10.1016/j.vibspec.2011.02.007]

Sarraguça MC, Cruz AV, Amaral HR, Costa PC, Lopes JA* (2011) Comparison of different chemometric and analytical methods for the prediction of particle size distribution in pharmaceutical powders, Anal. Bioanal. Chem., 399, 2137-2147 [doi:10.1007/s00216-010-4230-6]



  • Ricardo Páscoa

”Non-invasive near-infrared spectroscopic technology for early prediction and optimization of wine quality”, Faculty of Pharmacy, University of Porto, grant funded by FCT, Portugal (started Jan 1, 2012)

  • Mafalda Sofia Cruz Sarraguça

High-throughput spectroscopic characterization of solid forms and cocrystals: improving pharmaceutical manufacturing and enhancing pharmacological properties (since 2011)

  • Jorge Sarraguça

Mathematical modeling framework of flow analysis methods for chemical, biochemical and pharmaceutical applications (since 2009)

  • Clara Cecília Santana Sousa

(as co-supervisor):“Genotypic and high-throughput methodologies for tracing the emerging pathogen Escherichia albertii”, Faculty of Pharmacy, University of Porto, grant funded by FCT, Portugal (supervised by Luísa Peixe, Faculty of Pharmacy, University of Porto) (Jul 2011 – Mar 2014)

  • Paulo Roberto da Silva Ribeiro

“High-performance analytical methods for the spectroscopic characterization of pharmaceutical solid forms, polymorphs and co-crystals of antihypertensive drugs”, Faculty of Pharmacy, University of Porto, grant funded by FAPEMA, Brazil (March 2013 – March 2014).


  • Carla Patrícia da Silva Coutinho

Differentiation of clonal variants of the Burkholderia cepacia complex isolated during chronic respiratory infection in cystic fibrosis patients with FTIR spectroscopy (Faculty of Pharmacy/UP, 25 February 2011) (co-supervised by Isabel Sá-Correia IST/UTL)

  • Mafalda Sofia Cruz Sarraguça

Improved qualilty control of solid dosage forms for the pharmaceutical industry with high-throughput vibrational spectroscopy (Faculty of Pharmacy/UP, 6 December 2010)

  • Ornella Ewa Preisner

(as co-supervisor): Fingerprinting of pathogenic bacteria with Fourier-Transform Infrared spectroscopy (FTIR) and chemometrics (Instituto Superior Técnico/UTL, May 2009) (supervised by José Cardoso Menezes IST/UTL) (May 2009)

  • Catarina Pereira Miguel

(as co-supervisor) The red and the green: a study on the materials, techniques and meaning of the green and red colours in medieval Portuguese illuminations (Faculdade de Ciências e Tecnologia/UNL) (supervised by Maria João Melo FC/UNL, co-supervised by Mark Clark UAmsterdam) (Dec 2012)

  • Susana M. M. A. Lopes

(as co-supervisor):“Temas de carreira em sociedades de advogados: o contributo da adaptabilidade e dos factores cognitivos na identificação dos talentos. Um estudo internacional”, PhD in Psychology, Faculdade de Psicologia e Ciências da Educação, Universidade de Lisboa (supervised by Maria Assunção, Faculdade de Psicologia e Ciências da Educação, Universidade de Lisboa) (since 2011)

  • Bjorn-Hendrik Peters

(as co-supervisor):“Impact of protein production method parameters on the quality of lyophilized proucts”, PhD in Pharmaceutical Technology, Faculty of Health Sciences, University of Eastern Finland (supervised by Jarkko Ketolainen, Faculty of Health Sciences, University of Eastern Finland) (since 2012)

  • Ana Filipa Tavares Silva

“Application of Process Analytical Technology and Quality-by-Design tools to pharmaceutical continuous manufacturing processes”, PhD in Pharmaceutical Sciences, Faculty of Pharmacy, University of Porto and Faculty of Pharmacy of University of Ghent (co-supervised by Thomas de Beer, Faculty of Pharmacy, University of Ghent) (since 2012)

  • Miguel Pedro Estrada Lopo

“Winemaking process advanced monitoring and control with high-throughput vibrational spectroscopy for the ‘vinho verde’ wine industry: producing higher-valued wines and ensuring authenticity”, PhD in Pharmaceutical Sciences-Analytical Chemistry, Faculty of Pharmacy, University of Port, grant funded by FCT, Portugal o (since 2013)

  • Cláudia Andreia Teixeira dos Santos

“Oenological grapes potential: early control of grapes ripening towards the production of high-valued wines”, PhD in Pharmaceutical Sciences-Analytical Chemistry, Faculty of Pharmacy, University of Porto, grant funded by FCT, Portugal (since 2013)

  • João Botelho

(as co-supervisor): “…”, Faculty of Pharmacy, University of Porto, Portugal (supervised by Clara Santana Sousa and co-supervised by Luísa Peixe, Faculty of Pharmacy, University of Porto) (since 2013)


  • Sandra Sofia Mota Rodrigues

(as co-supervision): Matematical modelling of single interface flow analysis (SIFA) systems (Faculty of Pharmacy/UP) (supervised by José Costa Lima FF/UP, co-supervised by João Machado Santos FF/UP) (July 2009)

  • Sandra Maria Pontes Brogueira Lopes

Desenvolvimento acelerado de separações cromatográficas na indústria farmacêutica com métodos quimiométricos (Instituto Superior Técnico/UTL, May, 2007) (co-supervised by Maria Teresa Leal IST/UTL)

  • Ricardo Nuno Mendes de Jorge Páscoa

Monitorização in-situ de processos de tratamento de águas residuais com espectroscopia de infra-vermelho próximo (Faculty of Pharmacy/UP, December, 2006)

  • Mónica Cancela de Abreu Gonçalves Vaz Almeida Miranda

Aplicação da espectroscopia de FT-IR para a serotipagem e avaliação da susceptibilidade à penicilina em Streptococcus pneumoniae (Faculty of Medicine/UL, May 14, 2006) (co-supervised by Mario Ramirez FM/UL).

  • Raquel de Queiroz Portela Mendes de Figueiredo

“Manufacturing process and quality control of solid dosage forms optimization for the pharmaceutical industry by near infrared spectroscopy and chemometric analysis”, MSc in Industrial Pharmaceutical Chemistry, Faculty of Pharmacy, University of Coimbra, September 24, 2012 (co-supervised by Jorge Ribeiro Salvador, Faculty of Pharmacy, University of Coimbra) (18 Valores)

  • Raquel Maria Tenório da Silva

“High-throughput assessment of FTIR spectroscopy for morphological characterization of E. coli mutants”, MSc in Pharmaceutical Engineering, Faculty of Pharmacy, University of Lisbon, October 8, 2012  (co-supervised by Isabel Portugal, Faculty of Pharmacy, University of Lisbon) (16 Valores)

  • Rita Batista Matias

“In-situ drug dissolution tests monitoring with uv/vis/NIR spectroscopy”, MSc in Quality Control, Faculty of Pharmacy, University of Porto (Nov 2013)


2003 – CUF Award – Award assigned by CUF (Companhia União Fabril) and FCT (Fundação para a Ciência e Tecnologia) to the best Portuguese PhD Thesis in Chemical Engineering with industrial application produced between 1999 and 2001 Prémio CUF atribuído pela (honorable mention, EUR1000 prize)

2004 – Bioempreendedor 2004 Award – Award assigned by ICEP (Instituto das Empresas para os Mercados Externos) and APBio (Associação Portuguesa de Biotecnologia) to the best entrepreneurship project in the biotechnology area (Winner, EUR5000 prize)

2012 – Young Scientist Paper Award (First Place) – Awarded to Nídia Lourenço at the 6th International Conference on Environmental Science and Technology 2012 for the communication “Real Time Monitoring of a Sequencing Batch Reactor with In-Situ NIR Spectroscopy” (authors: Nídia Lourenço, Cláudio Fernandes, João Almeida Lopes and Helena Pinheiro).

Additional information

President of the directive board of ANICT-Associação Nacional de Investigadores em Ciência e Tecnologia (Jan 2013 – Jan 2014)

Member of the steering committee of the EUFEPS-PAT and QbD Network (since 2010)

Member of Ordem dos Engenheiros (Portuguese Engineers College) (since 1997)

Member of Sociedade Portuguesa de Química (Portuguese Society of Chemistry) (since 2007)

  1. Coffee Beans Quality Assessment book chapter | Chemometrics and Process Analytical Technology Unit

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